Biology – 6.1 Plant nutrition – photosynthesis | e-Consult
6.1 Plant nutrition – photosynthesis (1 questions)
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Effect of Temperature: The rate of photosynthesis increases with temperature up to an optimum temperature. Beyond this optimum, the rate decreases rapidly as enzymes involved in photosynthesis denature. This is because the enzymes responsible for the light-independent reactions are sensitive to temperature changes.
Experimental Conditions:
- Use a plant (e.g., *Elodea*) in a test tube filled with a dilute sodium bicarbonate solution.
- Maintain a constant carbon dioxide concentration (e.g., using a dilute sodium bicarbonate solution).
- Vary the temperature of the water bath in which the test tube is placed. Use a range of temperatures (e.g., 15°C, 20°C, 25°C, 30°C).
- Maintain a constant light intensity (e.g., using a lamp at a fixed distance).
- Measure the rate of photosynthesis by counting the number of oxygen bubbles produced per minute.
Measuring the Rate of Photosynthesis: The rate of photosynthesis can be measured by counting the number of oxygen bubbles produced per minute. Oxygen is a byproduct of the light-dependent reactions. The more oxygen bubbles produced, the faster the rate of photosynthesis.