Calculate magnification and size of biological specimens using millimetres as units.
2.2 Size of Specimens
What is Magnification? 📷
Magnification tells us how many times bigger the image of a specimen looks compared to the real specimen. Think of it like zooming in on a photo: the closer you zoom, the bigger the picture appears.
Formula (inline LaTeX): $M = \dfrac{I}{O}$ where $I$ = image size and $O$ = object size.
Finding the Size of a Specimen 🧪
If you know the magnification and the size of the image, you can work out the real size:
Formula (inline LaTeX): $O = \dfrac{I}{M}$
Example: A microscope image of a cell is 5 mm long and the magnification is 100×. Real size = 5 mm ÷ 100 = 0.05 mm (50 µm).
Step‑by‑Step Example 🧩
- Measure the image size with a ruler: 12 mm.
- Read the magnification from the microscope: 200×.
- Calculate real size: $O = 12\,\text{mm} ÷ 200 = 0.06\,\text{mm}$ (60 µm).
Tip: Always keep the units consistent (mm, µm, etc.).
Exam Tip Box 📚
Remember:
- Use the correct formula: $M = I/O$ or $O = I/M$.
- Check that you convert units if the answer is requested in micrometres (µm).
- Show all steps in your calculation; examiners look for clear reasoning.
Practice: If a specimen image is 3 mm and the magnification is 150×, what is the real size? (Answer: 0.02 mm or 20 µm).
Quick Reference Table 📊
| Scenario | Formula | Result |
|---|---|---|
| Image = 10 mm, Magnification = 50× | $O = I/M$ | $10\,\text{mm} ÷ 50 = 0.2\,\text{mm}$ |
| Object = 0.08 mm, Magnification = 200× | $I = O \times M$ | $0.08\,\text{mm} × 200 = 16\,\text{mm}$ |
Revision
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